Here are several New York City hotels that list a 24-hour business center (source links after each entry):

• The Plaza (Fifth Avenue / Midtown). — “Complimentary 24H Business Center access.” (fairmont.com)
• Grand Hyatt New York (Grand Central / Midtown East). — property listings advertise a “24‑hour business center.” (hotels.com)
• Hilton New York Midtown (Avenue of the Americas / Midtown). — booking listings note a “24‑hour business center.” (booking.com)
• Sofitel New York (45 W 44th St / Midtown). — multiple property listings show a “24‑hour business center.” (hotels.cn)
• Gramercy Park Hotel (Gramercy / Flatiron). — business center described with 24‑hour administrative staffing. (travel.usnews.com)

Note: hotel amenities and hours can change. I recommend calling the hotel or checking its official website to confirm current business‑center hours and any fees before you book. If you want, I can check a neighborhood (Times Square, Midtown, Financial District, etc.), specific dates, or add more hotels with 24‑hour business centers.